Resstome - Feb 1, 2024 · Drinking water distribution systems (DWDSs) are important for supplying high-quality water to consumers and disinfectant is widely used to control microbial regrowth in DWDSs. However, the disinfectant's influences on microbial community and antibiotic resistome in DWDS biofilms and the underlying …

 
Jul 25, 2018 · Additionally, there is a need to compare the resistant bacteria that can be grown with the overall resistome, which will provide a better measure of the ‘phantom resistome’, that is, the .... Herb and rye

Sep 8, 2022 · The metagenomic variations and the levels of the environmental impact of four different soil samples from the Amazon region were evaluated, emphasizing the resistome. Soil samples from the organic phase from the different forest, pasture, and transgenic soybean monocultures of 2–14 years old were collected in triplicate at each site.Jan 22, 2013 · The Resistome is defined as the collection of all genes that could contribute to a phenotype of antibiotic resistance (D’Costa et al., 2006; Wright, 2007, 2010; Forsberg et al., 2012). This is a purely functional definition made from a human and medical point of view, for as we have seen, the resistome encompasses … Join The Redstone Family Today Are you ready to join the credit union that puts you first? Become a member of the Redstone family and you’ll start enjoying the great rates, personalized financial advice, and excellent customer service Redstone is known for. Oct 3, 2013 · The serum resistome comprised 56 genes, the majority of which encode membrane proteins or factors involved in lipopolysaccharide (LPS) biosynthesis. Targeted mutagenesis confirmed a role in serum resistance for 46 (82%) of these genes. The murein lipoprotein Lpp, along with two lipid A-core biosynthesis enzymes WaaP and WaaG, were most strongly ...Sep 21, 2018 · Antibiotics and bacterial resistance. Antibiotics are the agents used commonly in the treatment and prevention of infections. Owing to their structure and degree of affinity to target sites, they are classified into Penicillin's, Cephalosporins, Tetracyclines, Aminoglycosides, Macrolides, Sulfonamides, Quinolones, Diaminopyrimidines, Polymyxin and Carbapenems (Sengupta et al., 2013; Bi et al ... Reserve your meeting space. Redstone is the perfect location for your next meeting or community event. We offer multiple conference room spaces to host your events with two locations in Huntsville, AL and one in Murfreesboro, TN. Catering options are available for all three locations. Whether it’s your monthly business networking group ... Jan 1, 2023 · There are a few hypothesized ways cyanobacteria may play a role in environmental AR and the resistome: through intrinsic and generally immobile ancient resistance mechanisms to antibiotics, the harboring of resistome-relevant mobile ARGs, or the promotion or inhibition of other bacteria that can host ARGs. …Feb 19, 2021 · The use of life-saving antibiotics has long been plagued by the ability of pathogenic bacteria to acquire and develop an array of antibiotic resistance mechanisms. The sum of these resistance mechanisms, the antibiotic resistome, is a formidable threat to antibiotic discovery, development, and use. The study and understanding of the molecular mechanisms in the resistome provide the basis for ... Must be eligible for membership and open a share savings account in order to join RFCU® and to open a “You Name It" Savings Account. Minimum balance of $5 is required to open and be maintained in the share savings account. Some restrictions apply. The minimum daily balance required to earn dividends is $500. No dividends will be earned for ... Fri 9:00 AM - 5:00 PM. Sat 9:00 AM - 1:00 PM. (800) 234-1234. https://www.redfcu.org. Redstone is a federally chartered credit union serving members North Alabama and Middle Tennessee. We work to help our members, their families, and our communities achieve their financial dreams. We offer checking, savings, loans, mortgages, credit cards ... Jan 23, 2019 · Disinfection regimes are considered the most solid strategy to reduce microbial risks in drinking water, but their roles in shaping the antibiotic resistome are poorly understood. This study revealed the alteration of antibiotic resistance genes (ARGs) profiles, their co-occurrence with mobile genetic elements (MGEs), and potential hosts during drinking water …Apr 12, 2022 · The overall presence of all resistance genes in a specific environment is defined as a resistome. Spatial proximity of surfaces and different hygienic conditions leads to the transfer of antibiotic resistant bacteria (ARB) within urban environments. Built environments, public transportation, green spaces, and citizens’ behaviors all support ... Sep 15, 2019 · Moreover, most studies focused on the changes of soil ARGs after manure application, and some important questions are urged to be solved, such as 1) the identification of the core (shared) soil ARGs with and without inorganic or organic fertilizer and their contribution to soil resistome, and 2) whether the change of soil …Nov 2, 2020 · There was a clear separation in resistome composition between samples from different ecological niches, with 12% of resistome variation explained at both the ARG type and gene levels (Adonis test ...Aug 1, 2018 · 1. Introduction. Antibiotics have been widely used to treat bacterial infections since the 1940s and have revolutionized global medicine (Allen et al. 2010; Marti et al. 2014).However, the widespread occurrence of microbial resistance to antibiotics has become ‘possibly the best example of human-driven evolution in …Feb 21, 2021 · The study and understanding of the molecular mechanisms in the resistome provide the basis for traditional approaches to combat resistance, including semisynthetic modification of naturally occurring antibiotic scaffolds, the development of adjuvant therapies that overcome resistance mechanisms, and the total synthesis of new antibiotics and the... The guts of urbanized people worldwide are known to contain less microbial biodiversity than those of humans living rurally. The worry is that the loss of key species contributes … Use the calculator below to help determine how much home you can afford, and to get your personal rate quote. Must be eligible for membership and open a share savings account to obtain a mortgage loan product. A $5 minimum balance is required to open and must be maintained in the share savings account at all times. Oct 3, 2013 · The serum resistome comprised 56 genes, the majority of which encode membrane proteins or factors involved in lipopolysaccharide (LPS) biosynthesis. Targeted mutagenesis confirmed a role in serum resistance for 46 (82%) of these genes. The murein lipoprotein Lpp, along with two lipid A-core biosynthesis enzymes WaaP and WaaG, were most strongly ... With Redstone’s online banking, you can do more with your money when it’s convenient for you. Manage. Account. View your account balance and activity. Review cleared checks (within the last 12 months) Open or upgrade an account (savings, checking, certificate, or money market account) Reorder your personalized checks. Unlike conventional loans, VA loans don’t require mortgage insurance (MI), since they are backed by the Department of Veterans Affairs. VA Loans usually offer more competitive rates, flexible underwriting, and no income limits. Plus, they offer up to 100% financing and lower closing cost fees for qualified buyers. It’s a win-win! Redstone Federal Credit Union. 19,291 likes · 21 talking about this. Redstone Federal Credit Union is the largest credit union in Alabama, serving over 750,000 members in Huntsville and the Tennessee... Locations. Our Guntersville and Fortress locations have moved. We look forward to seeing you at our new locations! With thousands of surcharge-free ATMs across the globe, Interactive Teller Services (ITMs), and 27 Redstone branches throughout the Tennessee Valley, we've got you covered. Redstone has joined several national and international ATM ... Passionné depuis 1993. Expédition en 24/48h. Aide et conseils 03 85 74 95 60. Depuis 1993, Restom est votre spécialiste français de la chimie appliquée à la restauration des …Jul 2, 2021 · The overuse of antibiotics in medicine and agriculture has contributed to the growing public health burden of antimicrobial resistance [].Studies have identified sets of antimicrobial resistance genes (ARGs) known collectively as the resistome [2, 3] in animals, humans, and the environment [4,5,6].The human gut microbiome is a critical reservoir of ARGs [7, 8], but …Mar 10, 2024 · A possible explanation for the changes in the resistome profiles between the surface water and sedimentary microbiomes is related to the fact that the resistance genes are carried by bacteria and a shift in bacteria community assemblages potentially resulted in a significant shift in the resistance genes profiles. Furthermore, due to the ...May 27, 2023 · The increased genetic repertoire available to this “mutator” parasite can be leveraged to drive P. falciparum resistome discovery. The ability to evolve Plasmodium drug resistance in vitro is ...Oct 14, 2021 · The resistome structure temporally evolved, and Acinetobacter and Pseudomonas were the most relevant carriers of ARGs. The most abundant ARGs were associated with resistance to β-lactams (27.2%), tetracyclines (26.9%), aminoglycosides (25.3%), and quinolones (7.5%), but the resistome composition evolved over time.Jun 4, 2022 · The resistome describes the array of antibiotic resistant genes (ARGs) present within a microbial community. Recent research has documented the resistome in the rumen of ruminants and revealed that the type and abundance of ARGs could be affected by diet and/or antibiotic treatment. However, most of these studies only assessed ARGs using …Better interrogation of antimicrobial resistance requires new approaches to detect the associated genes in metagenomic samples. Targeted enrichment is an ideal … Phone Number: (800) 234-1234. Report Phone Problem. Address: Redstone Federal Credit Union Scottsboro Branch 23149 John T Reid Parkway Scottsboro, AL 35768. Website: Visit Website. Online Banking: Redstone Federal Login. Feb 1, 2020 · There are growing concerns about the possible contributions of both manure and compost to increases of ARGs found in the soil resistome and in known pathogenic bacteria. It was shown that the relative abundance of β-lactam resistant bacteria was much higher in manure-amended soil than in unamended soil (Gou et al., 2018).Background The resistome, the collection of antibiotic resistance genes (ARGs) in a microbiome, is increasingly recognised as relevant to the development of …Sep 23, 2022 · Resistome analysis was performed on both full and rarefied data. Metagenome assemblies were compared using BLASTn against the CARD database with a cut-off of 80% identity and 70% coverage . Host ...Oct 23, 2023 · The resistome structure of external habitats differed significantly from the human fecal resistome, likely driven by biotic and abiotic factors in the various habitats. We subsequently identified a unique core resistome of human feces, which was extremely different from those in the other habitats, likely due to combinations of microbial ... Use the calculator below to help determine how much home you can afford, and to get your personal rate quote. Must be eligible for membership and open a share savings account to obtain a mortgage loan product. A $5 minimum balance is required to open and must be maintained in the share savings account at all times. The concept of the antibiotic resistome was introduced just over a decade ago, and since then, active resistome studies have been conducted. In the present study, we describe the previously established concept of the resistome, which encompasses all types of antibiotic resistance genes (ARGs), and t … Fri 9:00 AM - 5:00 PM. Sat 9:00 AM - 1:00 PM. (800) 234-1234. https://www.redfcu.org. Redstone is a federally chartered credit union serving members North Alabama and Middle Tennessee. We work to help our members, their families, and our communities achieve their financial dreams. We offer checking, savings, loans, mortgages, credit cards ... Sep 5, 2019 · The ARGs in the gut microbiota of infants can be traced back to the resistome present in breast milk microbiota. To get a better understanding of this phenomenon it is important to analyse the bacterial resistome from infant-mother pair, where the ARG signatures in infant gut can be compared with those present … The concept of the antibiotic resistome was introduced just over a decade ago, and since then, active resistome studies have been conducted. In the present study, we describe the previously established concept of the resistome, which encompasses all types of antibiotic resistance genes (ARGs), and t … Jul 6, 2022 · The poultry microbiome was the most diverse, harboring the highest number of different ARGs and contained the highest number of unique ARGs. So far, research has been relatively limited on the poultry resistome and how it compares to other livestock microbiomes (Ma et al., 2021), therefore this study sheds new light on the poultry resistome. Join Redstone today and see what’s possible for you. ARE YOU ELIGIBLE TO JOIN BECOME A MEMBER. Must be eligible for membership and open a share savings account to join RFCU. A $5.00 minimum balance is required to open and be maintained in the share savings account at all times. Fees and other restrictions may apply. Feb 27, 2023 · Resistome Tracker is a tool that can be used to examine the distribution of antimicrobial resistance genes in genomes deposited in NCBI (updated weekly).Apr 12, 2022 · The overall presence of all resistance genes in a specific environment is defined as a resistome. Spatial proximity of surfaces and different hygienic conditions leads to the transfer of antibiotic resistant bacteria (ARB) within urban environments. Built environments, public transportation, green spaces, and citizens’ behaviors all support ... Background Antimicrobial resistance is a major global health challenge. Metagenomics allows analyzing the presence and dynamics of “resistomes” (the …Jun 15, 2023 · The resistome profile of the observed pathogen of origin analysis was generated based on the hits obtained from the CARD database v3.2.4 (Alcock et al., 2020). 2.7. Statistical analysis.Dec 15, 2018 · Phyllosphere resistome. By using HT-qPCR, this study provides new insights into the antibiotic resistome in plant microbiome, as well as the factors driving the shift of antibiotic resistome in plant microbiome, which is critical for understanding the spread of ARGs in the environment and associated risks.Jan 1, 2024 · The skin antibiotic resistome after exposure overlapped with the dust antibiotic resistome and partially overlapped with the swine feces antibiotic resistome, suggesting that the distributions of skin and dust ARG profiles were relatively similar and differs somewhat from skin with swine feces. Diversity of the antibiotic resistome in students ...Sep 27, 2019 · The dynamic changes in the resistome are likely a result of gut microbiota assembly, which is closely associated with diet transition in dairy calves. Modifications in the resistome may be ...Jan 1, 2024 · Additionally, the resistome risk was analyzed by identifying the high-risk ARGs and their co-occurrences with MGEs and virulence factors on the same metagenomic contigs, and the sources of ARGs in the downstream were apportioned using the metagenomic resistome signatures and a source tracking tool. The study is of great significance to better ...Mar 24, 2021 · Over recent years, sophisticated and diverse pipelines have been established to facilitate raw data processing and annotation. Despite the progress, there are no easy-to-use tools for comprehensive visual, statistical and functional analysis of resistome data. Thus, exploration of the resulting large complex datasets remains a key …Jul 6, 2022 · The poultry microbiome was the most diverse, harboring the highest number of different ARGs and contained the highest number of unique ARGs. So far, research has been relatively limited on the poultry resistome and how it compares to other livestock microbiomes (Ma et al., 2021), therefore this study sheds new light on the poultry resistome.Sep 21, 2018 · Antibiotics and bacterial resistance. Antibiotics are the agents used commonly in the treatment and prevention of infections. Owing to their structure and degree of affinity to target sites, they are classified into Penicillin's, Cephalosporins, Tetracyclines, Aminoglycosides, Macrolides, Sulfonamides, Quinolones, Diaminopyrimidines, Polymyxin and Carbapenems (Sengupta et al., 2013; Bi et al ... The health risks of antibiotic resistome and pathogens on microplastic. Health risks associated with the antibiotic resistome are closely linked to the presence of MGEs and pathogenic hosts (Zhang et al., 2022). In the present study, significantly higher concentrations of MGEs were observed in the MPs than in the surrounding water.Jan 23, 2019 · Disinfection regimes are considered the most solid strategy to reduce microbial risks in drinking water, but their roles in shaping the antibiotic resistome are poorly understood. This study revealed the alteration of antibiotic resistance genes (ARGs) profiles, their co-occurrence with mobile genetic elements (MGEs), and potential hosts during drinking water …Sep 30, 2015 · An explanation for the limited dissemination of the WWTP core resistome could be that the resistance genes are not selected for or mobilized. However, studies have shown that even very low ...May 15, 2023 · The resistome features (ARG types, subtypes, indicator ARGs, and emerging mobilizable ARGs: mcr and tet(X)) in these habitats were benchmarked via a standardized workflow. We found that wastewater and wastewater treatment works were characterized to be reservoirs of more diverse genotypes of ARGs than any other habitats including human and ...Abstract. The human gut is home to a myriad of organisms. While some are harmless commensals, others are transient, pathogenic flora. The gut microbiome is composed of …Background Antimicrobial resistance is a major global health challenge. Metagenomics allows analyzing the presence and dynamics of “resistomes” (the …Jan 15, 2023 · Once the basic traits of antibiotic resistome (e.g., abundance and richness of ARGs) in the examined HWTSs were unraveled, we further asked how the key traits closely related to the environmental spread (e.g., genetic mobility) and human health risk (e.g., host pathogenicity and multi-resistance) of wastewater …Jan 15, 2022 · The air-borne resistome is a complex system which is part of the environmental resistome with multiple sources, fate processes and sinks. Therefore, studies focusing on source tracking and apportionment are required to improve our understanding of the occurrence, reservoirs, dissemination, and fate of ARB and ARGs in the air-borne resistome. (2)Jan 22, 2013 · The Resistome is defined as the collection of all genes that could contribute to a phenotype of antibiotic resistance (D’Costa et al., 2006; Wright, 2007, 2010; Forsberg et al., 2012). This is a purely functional definition made from a human and medical point of view, for as we have seen, the resistome encompasses diverse genes whose original ...Feb 17, 2021 · Here, we report the first comprehensive large sample size analysis of the human skin resistome. The gut resistome mainly includes genes conferring resistance against tetracyclines, ß-lactams, aminoglycosides, and glycopeptides, followed by chloramphenicol and macrolides . For the lung, the most abundant ARGs are ß-lactamases .Aug 16, 2017 · The transcriptional patterns of a core resistome over the course of composting were identified, and microbial phylogeny was the key determinant in defining the varied transcriptional response of resistome to this dynamic biological process. This research demonstrated the benefits of composting for manure …Here, we study the resistome of 771 samples from five major body parts (skin, nares, vagina, gut, and oral cavity) of healthy subjects from the Human Microbiome …Jan 15, 2023 · Once the basic traits of antibiotic resistome (e.g., abundance and richness of ARGs) in the examined HWTSs were unraveled, we further asked how the key traits closely related to the environmental spread (e.g., genetic mobility) and human health risk (e.g., host pathogenicity and multi-resistance) of wastewater …Nov 28, 2023 · Concerning the resistome of bla and qnr, the prevalence rates between chickens and patients with CDI were the most similar (R2 = 0.46); blaTEM, blaOXA, blaCTX-M, and qnrS were highly prevalent in ...Jan 20, 2024 · Conclusion: MetaCompare 2.0 successfully ranked a wide array of environments according to both human health and ecological resistome risks, with both scores being strongly impacted by anthropogenic stress. We packaged the improved pipeline into a publicly-available web service that provides an easy-to-use interface for computing resistome risk ...parabasis. (in classical Greek comedy ) an address from the chorus to the audience. Mar 18, 2024. Mar 18, 2024. Mar 17, 2024. Mar 08, 2024. RESISTOME …Jan 20, 2006 · Synercid, which was FDA-approved in 1999 for the treatment of drug-resistant bacteremia, is a combination of two semisynthetic derivatives of Streptomyces metabolites, each with a distinct mode of action. Eighteen percent of resistant isolates tested were able to detoxify both antibiotics.Jan 20, 2024 · Here we introduce MetaCompare 2.0, which incorporates several improvements to address the above-noted limitations of MetaCompare 1.0 and other ARG risk ranking approaches. Specifically, we introduce two distinct resistome risk scores, one corre-sponding to the ecological resistome risk (ERR) and the other … The human gut is home to a myriad of organisms. While some are harmless commensals, others are transient, pathogenic flora. The gut microbiome is composed of diverse bacterial flora, and apart from playing a major role in protecting from various infectious and non-infectious diseases, it plays an important role in resistance to antimicrobials. Sep 21, 2018 · Antibiotics and bacterial resistance. Antibiotics are the agents used commonly in the treatment and prevention of infections. Owing to their structure and degree of affinity to target sites, they are classified into Penicillin's, Cephalosporins, Tetracyclines, Aminoglycosides, Macrolides, Sulfonamides, Quinolones, Diaminopyrimidines, Polymyxin ...Feb 9, 2023 · Application of reprogrammed bacteriophage to functional metagenomics in clinically relevant bacterial strains improves identification of antibiotic resistance genes, including those against ... Redstone Federal Credit Union headquarters is in Huntsville, Alabama has been serving members since 1951, with 27 branches and 69 ATMs. The Main Office is located at 220 Wynn Drive, Huntsville, Alabama 35805. Contact Redstone at (800) 234-1234. Access Redstone Federal Login, hours, phone, financials, and additional member resources. Jan 9, 2024 · In this study, we found that antibiotic treatment led to destabilization of the gut microbiome and resistome structural communities, exhibiting a greater impact on the resistome than on the microbiome. The composition of the gut resistome at various developmental stages was influenced by the abundance and richness of different core …The global threat of antimicrobial resistance has driven the use of high-throughput sequencing techniques to monitor the profile of resistance genes, known as …Sep 15, 2019 · Moreover, most studies focused on the changes of soil ARGs after manure application, and some important questions are urged to be solved, such as 1) the identification of the core (shared) soil ARGs with and without inorganic or organic fertilizer and their contribution to soil resistome, and 2) whether the change of soil …Feb 13, 2015 · Model 2 was beta tested on independent sites and explained over 78% of the variation in integron prevalence showing a significant predictive ability. We believe all models in this study are highly ...Jan 20, 2024 · Conclusion: MetaCompare 2.0 successfully ranked a wide array of environments according to both human health and ecological resistome risks, with both scores being strongly impacted by anthropogenic stress. We packaged the improved pipeline into a publicly-available web service that provides an easy-to-use interface for computing resistome risk ...

Aug 1, 2018 · 1. Introduction. Antibiotics have been widely used to treat bacterial infections since the 1940s and have revolutionized global medicine (Allen et al. 2010; Marti et al. 2014).However, the widespread occurrence of microbial resistance to antibiotics has become ‘possibly the best example of human-driven evolution in …. Old men

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Welcome to resstome.com, your ultimate guide to the world’s best restaurants. Our goal is to provide food lovers with in-depth reviews, inspiring articles, …Jun 27, 2023 · Resistome composition was extracted when assessed by comparing total resistance gene abundance at baseline and following treatment as well as between placebo and treatment groups using dissimilarity matrices calculated by the Bray–Curtis . Relative abundance of antibiotic-specific genes following treatment …Jan 20, 2024 · Here we introduce MetaCompare 2.0, which incorporates several improvements to address the above-noted limitations of MetaCompare 1.0 and other ARG risk ranking approaches. Specifically, we introduce two distinct resistome risk scores, one corre-sponding to the ecological resistome risk (ERR) and the other …Oct 14, 2021 · The resistome structure temporally evolved, and Acinetobacter and Pseudomonas were the most relevant carriers of ARGs. The most abundant ARGs were associated with resistance to β-lactams (27.2%), tetracyclines (26.9%), aminoglycosides (25.3%), and quinolones (7.5%), but the resistome composition evolved over time.Jul 6, 2022 · The poultry microbiome was the most diverse, harboring the highest number of different ARGs and contained the highest number of unique ARGs. So far, research has been relatively limited on the poultry resistome and how it compares to other livestock microbiomes (Ma et al., 2021), therefore this study sheds new light on the poultry resistome.The term “antibiotic resistome” was first coined in 2006 by Gerry Wright’s group, who defined the soil resistome as “resistance determinants present in the soil”, … Redstone Discounts! & Visa Discounts: You must have a RFCU Visa debit or credit card to participate in Redstone Discounts! and Visa Discounts. Restrictions may apply. Discounts are provided by participating merchants. RFCU does not warrant, guarantee, or insure any product, purchase, or service offered by participating merchants. Sep 27, 2019 · The dynamic changes in the resistome are likely a result of gut microbiota assembly, which is closely associated with diet transition in dairy calves. Modifications in the resistome may be ...Jan 23, 2024 · The One World, One Health concept implies that antibiotic resistance (AR) in the soil-microbe-plant resistome is intricately linked to the human resistome. However, the literature is mainly confined to sources and types of AR in soils or microbes, but comprehensive reviews tracking AR in the soil-microbe-plant resistome are limited. The present review applies the … About Redstone Federal Credit Union. Redstone Federal Credit Union was chartered on Jan. 1, 1951. Headquartered in Huntsville, AL, it has assets in the amount of $3,744,190,658. Its 373,833 members are served from 24 locations. Deposits in Redstone Federal Credit Union are insured by NCUA. May 18, 2020 · Determining the effect of wastewater treatment in water resistome is a topic of interest for water quality, mainly under re-use and One-Health perspectives. The resistome, the plasmidome, and the ...Jun 15, 2023 · The resistome profile of the observed pathogen of origin analysis was generated based on the hits obtained from the CARD database v3.2.4 (Alcock et al., 2020). 2.7. Statistical analysis.Dec 15, 2018 · Phyllosphere resistome. By using HT-qPCR, this study provides new insights into the antibiotic resistome in plant microbiome, as well as the factors driving the shift of antibiotic resistome in plant microbiome, which is critical for understanding the spread of ARGs in the environment and associated risks. Reserve your meeting space. Redstone is the perfect location for your next meeting or community event. We offer multiple conference room spaces to host your events with two locations in Huntsville, AL and one in Murfreesboro, TN. Catering options are available for all three locations. Whether it’s your monthly business networking group ... May 5, 2022 · The standardized direct effects from microbial community on the changes of antibiotic resistome and MGEs were both 0.68 (p < 0.01), and then, MGEs directly impacted the changes of antibiotic resistome by 0.101. The effects of environmental variables on the antibiotic resistome were mostly indirect through the microbial community (0.383)..

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